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Pol J Cosmetol 2010, 13(1): 48-53pl,en  Free radicals properties of dietary supplements applied in cosmetologySławomir Wilczyński 1/, Magdalena Zdybel 1/, Anna Deda 1,2/, Ewa Pierzchała 2/ 1/ Katedra i Zakład Biofizyki, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej, Śląski Uniwersytet Medyczny, Sosnowiec 2/ Zakład Medycyny Estetycznej, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej, Śląski Uniwersytet Medyczny, Katowice Summary Introduction. Free radicals play an important role in the pathogenesis of many diseases. Some of dietary supplement may have protective properties against free radicals. Preventive efficiency of dietary supplements depend on antioxidants contents. To the most popular dietary supplements preparation containing plant and fish oils belong to. Aim. The aim of this work was to determine antiradical efficiency of four dietary supplements by use the EPR technique. Materials and methods. Antioxidative potential of selected dietary supplements was studied with the aid of electron paramagnetic resonance spectroscopy at X-band microwave frequencies (9.3 GHz). After adding to analyzed preparations the standard solution of free radicals (DPPH) the EPR spectra parameters were estimated. Amplitudes (A), linewidths and integral intensities (I) of the spectra were determined. Oenothera oil (OO), sardine fish oil (SFO) and two different samples of oli from greenland shark liver (OGS 1 and OGS 2) were analyzed. Results. All studied dietary supplements are effective in free radicals scavenging. In the decreasing free radicals scavenging capacity, the order was: OSG 1, SFO, OSG 2 and OO. No significant changes of linewidths were stated. Conclusions. It was proven that EPR technique is suitable to antioxidative properties of the studied dietary supplements determination. Obtain results may indicate that oils of animal origin are more efficient in free radicals scavenging in comparison with dietary supplements based on oils of plant origin. Key words: dietary supplements, antioxidants, electron paramagnetic resonance spectroscopy |