Evaluation of antioxidant properties of Sea buckthorn (Hippophaë rhamnoides L.) and European cranberry (Vaccinium oxycoccos L.) extractsMonika Michalak 1/, Ryszard Glinka 1/, Paweł Ramos 2/, Barbara Pilawa 2/ 1/ Społeczna Akademia Nauk w Łodzi 2/ Katedra i Zakład Biofizyki, Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej, Śląski Uniwersytet Medyczny w Katowicach Summary Introduction. Free radicals are extremely reactive molecules the activity of which leads to adverse changes in a human body and has negative influence on skin condition and functioning. The knowledge about harmfulness of free radicals induces to search for components supporting the natural mechanisms of antioxidant defense of organism. Due to the attributed ability to remove free radicals plant extracts are often used in cosmetic industry. Both Sea buckthorn and European cranberry are the objects of numerous studies, not only for their valuable chemical composition, but also because of their antioxidant potential as well as their wide application possibilities in cosmetology. Aim. The aim of this study was to examine and compare the antioxidant properties of Sea buckthorn and European cranberry. Material and methods. In this study glicolic extracts of Sea buckthorn (Hippophaë rhamnoides L.) and European cranberry (Vaccinium oxycoccos L.) fruits were used. The antioxidant properties of the plant extracts were tested using Electron Paramagnetic Resonance (EPR) method with the use of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. Results. The investigated plant extracts are characterized by different antioxidant properties. The higher ability to scavenge DPPH radical characterizes the Sea buckthorn extract. No EPR line was observed in the investigations even at maximum reinforcement. The EPR spectrum of the European cranberry has smaller amplitudes of EPR lines than in the reference sample, which shows that it has antioxidant properties but they are considerably weaker than these of Sea buckthorn. Conclusion. The obtained results indicate that it is possible to use Sea buckthorn and European cranberry in cosmetology as antioxidizing components. It was demonstrated that EPR spectroscopy is an effective method allowing to determine the antioxidant potential of plant extracts. Key words: antioxidants, Sea buckthorn, European cranberry, free radicals, DPPH |