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Copyright @ Pol J Cosmetol
Pol J Cosmetol 2014, 17(4): 296-302pl  Effect of laser biostimulation on free radicals formation in the skinAnna Krzeszewska-Zaręba, Magdalena Zdybel, Barbara Pilawa Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej w Sosnowcu, Śląski Uniwersytet Medyczny w Katowicach, Katedra i Zakład Biofizyki Summary Introduction. Laser biostimulation causes therapeutic effects in skin and other tissues. Absorption of laser radiation may lead to formation of free radicals in biological system, especially in melanin. Aim. The aim of this work was to determine concentration and properties of free radicals in melanin exposed to irradiation with laser of low power. Material and method. Free radicals were examined by the use of an X-band (9.3 GHz) electron paramagnetic resonance (EPR) spectroscopy. Examined melanin was bought in Sigma-Aldrich corporation. Melanin was prepared by oxidation of tyrosine with hydrogen peroxide. Result. The strong increase of o-semiquinone free radical concentration in melanin after laser irradiation during 20 minutes was stated. The shorter exposition to laser irradiation did not cause considerable free radical formation in the sample. Strong dipolar interactions and slow spin-lattice relaxation processes exist in melanin before and after laser irradiation. Conclusion. Laser irradiation cause free radical biostimulative effects in skin with participation of melanin. Key words: laser biostimulation, electron paramagnetic resonance (EPR) spectroscopy, free radicals |