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Copyright @ Pol J Cosmetol
Pol J Cosmetol 2018, 21(4): 336-342pl  Antioxidant activity of pomegranate (Punica granatum L.) extracts as a raw material for cosmetics applicationsAnna Nowak 1/, Joanna Zielonka-Brzezicka 1/, Adam Klimowicz 1/, Daria Wira 1/, Daria Wysocka 2/, Laura Synowiec 2/ 1/ Katedra i Zakład Chemii Kosmetycznej i Farmaceutycznej Pomorskiego Uniwersytetu Medycznego w Szczecinie 2/ Studenckie Koło Naukowe przy Katedrze i Zakładzie Chemii Kosmetycznej i Farmaceutycznej Pomorskiego Uniwersytetu Medycznego w Szczecinie Summary Introduction. The active ingredients in cosmetics are often plant-derived. Some fruits, such as pomegranate (Punica granatum L.), are a source of antioxidants, enzymes and minerals. The activity of fruits is based on their moisturizing, peeling, nourishing and brightening effects. Moreover, the contained antioxidants can contribute to reduce skin ageing. Material and methods. Antioxidant activity was determined by DPPH and FRAP method, while total polyphenol content by the Folin-Ciocalteu method. As extraction solvents 70% (v/v), 96% (v/v) ethanol and 99,8% (v/v) methanol were used. The extracts for analysis were prepared using ultrasound bath for 15, 30 and 60 minutes. Results. The antioxidant activity of pomegranate extracts, evaluated with the DPPH method, ranged from 1,80±0,05 mg trolox/g raw material for extract of seeds in 70% (v/v) ethanol (15 minutes) to 4,01±0,02 mg trolox/g raw material for methanol in extract of fruit skin (60 minutes). The ability to reduce ferric ions evaluated with FRAP method ranged from 0,55±0,03 mg FeSO4/g raw material for seeds, prepared in 70% (v/v) ethanol for 15 minutes to 3,59±0,20 mg FeSO4/g raw material for fruit skin extracted in methanol for 60 minutes. The highest total polyphenol content was found for fruit skin extracted with methanol, for 60 minutes - 5,81±0,04 mg gallic acid/g raw material. A positive correlation between the results obtained with the applied methods has been found. Conclusion. The alcoholic extracts of pomegranate fruits, showed a high antioxidant potential. The highest values, in most cases, were found for methanolic extracts extracted 60 minutes. High antioxidant activity of pomegranate fruits suggests the possibility to apply them as a valuable source of antioxidant in cosmetic preparations. Key words: pomegranate, DPPH, FRAP, Folin-Ciocalteu, alcoholic extracts |